AICRP on Nutrional and Physiological Interventions
for Enhancing Reprocitive Performance in Animals
Documentation of current status/extent of infertility and various causes of reproductive failures in animals
A survey was carried out in nine different states (Assam, Odessa, Karnataka, Tamil Nadu, Maharashtra, Gujarat, Rajasthan, Haryana, Uttar Pradesh) representing various agro-eco zones of the country to assess the infertility status of animals. About 65000 animals’ data had been collected to find out the extent of reproductive problems throughout the country.
Synchronization for estrus induction
ü The reproductive efficiency of cattle and buffaloes having reproductive problems was improved through estrus induction and timed AI using the different synchronization protocols. The average conception rate was increased up to 68% (ranging from 39 to 75%) by treating almost 1700 cows and buffaloes. In case of cows, the ovsynch and estradoublesynch and in case of buffalo estradoublesynch were found better than other protocols. Considering both the species, estradoublesynch could be used for improving the conception rate and thus enhanced the reproductive efficiency in cattle and buffaloes.
v Nutritional interventions to ameliorate infertility and improve reproductive efficiency
ü It was evident that most of the animals with reproductive problems had low energy and protein intake in diet with inadequate mineral supply. Accordingly, asupplement was formulated comprising of source of easily available energy, rumen degradable and non-degradable source of protein, most critical minerals etc. The milk fat increased to 3.85 % (range 3.5 to 4.2%) from 2.58% (range 2.5 - 3.0%) after 20 days of feeding the supplement. All the reproductive disordered animals supplemented with this specific feed formulation came to estrus (100%) and 76% of animals become pregnant.
v Developed Markers for predicting fertility status
ü Quantitative proteomics revealed differences in number and types of proteins upregulated in good and poor quality semen samples
ü Higher proportion of immune regulatory protein in low fertile bulls could determine the fertility status of a male.
v Developed an extender for semen cryopreservation
ü Some biomolecules have been identified as suitable semen extenders to improve post-thaw semen quality and that had cryoprotective effect on buffalo spermatozoa and improved field fertility.
v Developed mineral mixture for breeding bull
ü Bull mineral mixtures developed by ICAR-NIANP improved semen production and post thaw semen quality in breeding bulls.
ü The developed mineral mixture for breeding bulls was validated in bulls of BAIF, Pune and ICAR-CIRB, Hisar cattle and buffalo farms, respectively. The spermatozoa count was observed higher in bulls supplemented with this specific mineral mixture which resulted in more number of frozen semen straws per bull. The average sperm concentration was 1.32 x 109 /ml of semen. The average number of straws per ejaculate was 400 and average number of straws per bull was 47520. The conception rate using the straws made of this semen was 55%. The technology is cost-effective in terms of low cost of mineral mixture and more numbers of semen straws per bull.
Synchronization treatment of reproductive disordered cows; subsequently the cow conceived and given birth of calf
Feed supplement distributed to farmers Animal is examined for estrus symptom
‘NIANP-BULLMIN’-Mineral Mixture for Breeding Bull