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                                             Outreach Project on

Estimation of Methane Emission under Different Feeding Systems and Development of Mitigation Strategies

Date/Year of Start: 2008-2012 (XI plan); 2012-2017 (XII plan) & 2017-2020

Project Coordinator: Dr. Raghavendra Bhatta, Director

Principal Investigator: Dr P. K. Malik

Co-Investigator: Dr. A. P. Kolte

Participating Centres: XII Plan

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                                                                                  2017-2020

 

Salient Achievements

Enteric Methane Emission Database

An inventory on state wise enteric methane emission from Indian livestock was developed under outreach project. ICAR-NIANP estimate revealed that Indian livestock annually emits about 9.25 Tg enteric methane.

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The hotspots for enteric methane emission were also identified which needs urgent attention for the amelioration

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In vitro screening of phyto-sources for methane production

Methane production potential of more than 1000 feedstuffs including straws, tree leaves, shrubs, pasture grass, grain, oil cakes, agro-industrial by-products and compounded feeds from different agro-eco zones were determined in vitro and based on the methane production, the promising sources were selected and studied with graded level of supplementation for optimizing the level of inclusion in animal diet to curtail methane emission

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Equation for predicting methane production

Based on methane production potential and chemical composition of feedstuffs, an equation was developed to predict methane production from the given feed resources/feeding regimes

Methane (ml/100 mg digested substrate) = 14.346+0.010*CP*NDF-0.069*ADF-0.001*ADL-0.136*IVDMD

Development of methane ameliorative measures

Based on the in vitro and subsequent in vivo studies in large and small ruminants, various methane ameliorative measures were developed

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Tropical tree leaves reduce enteric methane emission

Selected tropical tree leaves from Azadirachta indica, Ficus religiosa and Artocarpus integrifolia were evaluated for their methane suppressing effect. Enteric methane was quantified using SF6 technique. Results from the in vivo study in sheep showed a reduction (p<0.05) of 20.5, 20.7 and 26.2% with the inclusion of Jack, Neem and Banyan tree leaves, respectively. 

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Tamarind (Tamarindus indica) seed husk as methane suppressant

Based on the in vitro results, tamarind seed husk at two graded levels of 2.5 and 5.0% of straw based diet was evaluated for methane reduction in crossbred cattle. Enteric methane emission was measured by sulphur hexafluoride (SF6) tracer technique. The supplementation of tamarind seed husk at 5% level decreased methane emission by 17% in cattle; however, supplementation at 2.5% did not show any significant reduction in methane emission.

Individual Vs Combo supplementation of tannins and saponins phyto-sources

The studies carried out using tannins or saponins as methane suppressants are confined to in vitro conditions only and there is limited information available on the in vivo methane amelioration. In vivo study was conducted in adult crossbred cattle to test whether combined supplementation of tamarind seed husk (CT source) and soapnut (saponin source) induce additional enteric methane reduction over and above the individual supplementation of selected sources. Results from the study revealed a significant (p<0.05) reduction (19.2%) in enteric methane emission (g d-1) on tamarind seed husk supplementation. However, the enteric methane emission (g/d) in soapnut (T2) and combo supplementation (T3) groups did not differ (p>0.05) from the control.

Silkworm pupae oil: a potent inhibitor of rumen methanogenesis

The research work has been initiated at the institute to explore the properties of silkworm (Bombyx mori) pupae oil as methane mitigating agent and optimize the level of the inclusion in animal feed in order to achieve significant methane reduction without compromising the feed fermentation. Silkworm pupae oil was supplemented with graded levels of 2 to 20% in finger millet and concentrate based diet. Our results indicated 15-50% reduction in methane production in vitro; while there was a concurrent substantial reduction in feed fermentation characteristics beyond the 4% level of silkworm pupae oil. The reduction in methane production at 2 and 4% level as compared to control basal diet was 16 and 30%, respectively.

Patent filed

  1. Enteric methane emission reduction using silkworm (Bombyx mori) pupae oil” patent application numbered 201641027475 dated 11-08-2016.
  2. Methane reduction using tamarind (Tamarindus indica) seed husk” patent application numbered 3475/CHE/2014
  3. Methane reduction using plant secondary metabolites” patent application numbered 2954/CHE/2012 dated 20.07.2012

Technology/product development

An anti-methanogenic feed supplement “Harit Dhara” was developed after rigorous in vitro and in vivo studies in different ruminant species. The supplementation of Harit Dhara at prescribed level in cattle, buffalo and sheep helps in reducing enteric methane emission in the tune of 18-20% and also helpful in improving productive performance of livestock. The use of Harit Dhara in large animals may also cut down the emission that would be equivalent to 700 litres CO2 in a day.  

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2. Climate Change Led Abiotic and Biotic Stress In Farm Animals and Amelioration With Nutritional
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1. Technology smart interventions
for doubling livestock farmers'
income: Strategies and Approaches.

2. Climate Change Led Abiotic and Biotic Stress In Farm Animals and Amelioration With Nutritional
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3. Recent Advances in Nutritional-Physiological Approaches for Improving Reproduction and Production in Livestock Under Climate Change Scenario.

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1. Technology smart interventions
for doubling livestock farmers'
income: Strategies and Approaches.

2. Climate Change Led Abiotic and Biotic Stress In Farm Animals and Amelioration With Nutritional
and Physiological Approaches.

3. Recent Advances in Nutritional-Physiological Approaches for Improving Reproduction and Production in Livestock Under Climate Change Scenario.

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1. Technology smart interventions
for doubling livestock farmers'
income: Strategies and Approaches.

2. Climate Change Led Abiotic and Biotic Stress In Farm Animals and Amelioration With Nutritional
and Physiological Approaches.

3. Recent Advances in Nutritional-Physiological Approaches for Improving Reproduction and Production in Livestock Under Climate Change Scenario.

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Upcoming Short Courses On

1. Technology smart interventions
for doubling livestock farmers'
income: Strategies and Approaches.

2. Climate Change Led Abiotic and Biotic Stress In Farm Animals and Amelioration With Nutritional
and Physiological Approaches.

3. Recent Advances in Nutritional-Physiological Approaches for Improving Reproduction and Production in Livestock Under Climate Change Scenario.

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Upcoming Short Courses On

1. Technology smart interventions
for doubling livestock farmers'
income: Strategies and Approaches.

2. Climate Change Led Abiotic and Biotic Stress In Farm Animals and Amelioration With Nutritional
and Physiological Approaches.

3. Recent Advances in Nutritional-Physiological Approaches for Improving Reproduction and Production in Livestock Under Climate Change Scenario.

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Upcoming Short Courses On

1. Technology smart interventions
for doubling livestock farmers'
income: Strategies and Approaches.

2. Climate Change Led Abiotic and Biotic Stress In Farm Animals and Amelioration With Nutritional
and Physiological Approaches.

3. Recent Advances in Nutritional-Physiological Approaches for Improving Reproduction and Production in Livestock Under Climate Change Scenario.

Details


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