The laboratory is established and mandated for doing research on micronutrients for animal production and reproduction. The laboratory is equipped with high throughput instruments and equipments for analyzing macro and micro minerals as well as toxic heavy metals in feeds and fodders, soil, water, animal products like meat, milk, egg, hair and skin etc. The recent research investigations carried out in the laboratory are mentioned below:
The laboratory is well equipped with all modern equipment for estimation of toxic elements in feeds, fodder water, soil and biological materials. The laboratory has HPLC system, thin layer chromatography facilities for estimation of toxins like the aflatoxins (B1, B2, G1 G2). Facilities also exists for estimation of fluorides, nitrate, lead and cadmium in different samples.
The laboratory is having all the facilities for proximate principles estimation and in vitro digestibility studies. It is well equipped with automated instruments like moisture analyzer, soxtherm, Kjeltec system, rapid fiber analyzer for rapid and precise estimation of various parameters like moisture, fat, protein and fiber. The laboratory caters to analytical requirements of various on going projects and the samples from outside agencies like milk federations, commercial firms and farmers.
The laboratory is established to isolate and characterize the rumen microbes including bacteria, protozoa and rumen methanogens. The laboratory is well equipped with modern facilities such as anaerobic chamber, fluorescent microscope, PCR, qPCR, gel doc, sample concentrator, bead beater etc.
The recent research in the laboratory is outlined below:
The Radioisotope & Endocrinology Laboratory was established in 1998 and certified RIA Type II Laboratory and licensed by AERB(BARC), , Mumbai to handle radioisotopes up to 2 millicurie activity. The objective of the laboratory is to carter the application of radioisotopes and molecular endocrinological tools for various research projects taken up at the institute. The laboratory is focused to conduct the basic endocrine and neuroendocrine hormone dependent productive status, wellbeing in farm animals and poultry. The laboratory is well equipped with state of art facilities to carry out assays, and monitor radiological safety aspects.
The recent research investigations carried out in the laboratory are as follows:
The laboratory is established for quantifying greenhouse gas emissions from the livestock production in the country and devising cost effective and farmers’ friendly mitigation strategies for reducing the emissions. The laboratory is a state of art facility, fully equipped for quantifying in vitro and in vivo methane emission, exploring rumen microbial diversity including methanogens and feed analysis. The laboratory has international collaborations with Japan, Germany, Australia and Kenya. At present, there are two ongoing international collaborative projects entitled “ Methane emission and its mitigation (ILRI-ICAR)” & “Optimized use of feed resources for high lifetime productivity of dairy cows and consequences on methane release (Indo-German)”. An international collaborative project (Indo-Japan) on “Methane mitigation using unexplored phyto-sources in ruminants and their effect on rumen microbial diversity” has recently been accomplished with Shinshu University, Ina and National Institute of Livestock and Grassland Science. The laboratory received the funding from ICAR, DBT and DST and handling a mega project on “Estimation of methane emission under different feeding systems and development of mitigation strategies” since 2007.
The recent research and accomplishment of the laboratory are outlined below:
Reproductive Physiology Laboratory focuses on improving fertility in farm animals by working on novel areas such as, profiling of sperm transcripts for predicting/screening male fertility, sperm sexing by proteomic approaches, molecular markers for assessment of semen quality and cryo-susceptibility, and spermatogonial stem cell research. The laboratory is equipped with modern state of art facilities such as Computer assisted semen analyzer (CASA), Controlled rate semen bio-freezer, Phase contrast and Fluorescent microscopes, Real-time PCR, 2D Gel electrophoresis, Chemi-Documentation system, Cryotome, Histotome, Cell culture incubator, Viscometer, Small volume spectrophotometer, Nucleic acid shearing sonicator, etc., for advanced semen evaluation techniques and sperm transcriptomics, proteomic and cryopreservation studies.
Many students from IVRI-Bareilly, KVAFSU-Bidar, AAU-Assam and Jain University-Bengaluru successfully completed their Master and Doctoral research thesis work at this Laboratory. The laboratory hosts FOCARS professional attachment training for ICAR scientists and DBT-TWAS fellowship program for international scholars. Presently, many research (Post-doc, Doctoral and Master) scholars are carrying out their research work at this laboratory.
The ongoing research projects in the laboratory are:
This laboratory was established in the year 2000. The laboratory produced the first buffalo embryo using oocyte retrieved from in vitro grown preantral follicles.
Work involves following aspects:
The Molecular Biology Laboratory of the institute was established in 1998 with the vision of conducting research in the critical and unresolved areas of reproductive biology, physiology, biotechnology and allied subjects in domestic animal species using advanced molecular biology tools and platforms. The laboratory has state of the art facilities for working in the area of protein, nucleic acid, recombinant protein production in prokaryotic and eukaryotic host and other in vitro experiments. The laboratory is equipped with ultracentrifuge, refrigerated and non-refrigerated centrifuges, ultrasonic processor, tissue homogenizer, gel electrophoresis system for proteins and nucleic acids separation, two-dimensional gel electrophoresis system, imaging systems for chromogenic, fluorescence and radio-labelled gels, fast pressure liquid chromatography system, CO2 incubators, Biological Safety cabinet, Laminar Air flow system, Inverted Fluorescence Microscope, DIC and fluorescent compound microscopes, shaker incubators, gradient and real-time PCR systems, spectrophotometer, micro-plate reader for small volume quantification, heavy duty Water purification system etc. This laboratory has on-going and envisaged research focuses as well as work in collaboration with intra and inter-institutional projects. Following are the current interest of research in the laboratory:
The laboratory was established in 2012 for unraveling the mechanism(s) of nutrient kinetics and the biophysical translation in integrating the homeostatic mechanism involved in the regulation of various physiological processes in livestock. The laboratory is equipped to handle gene expression studies, cell culture, hormone estimations and blood biochemical variables. The recent research investigations carried out in the laboratory are mentioned below:
The laboratory was established 2013 to serve the stakeholder through evaluation of feed resources and development of technologies related to feeds and fodder. The latest technologies are used to develop informatics tools to analyse data and disseminated the information on feeds and fodder to the farmers, policy makers, feed, dairy and poultry industry. Nanotechnology is emerging field, its application in feed industry is vital in coming year. Hence, nanotechnology related research works are carried out in this laboratory. Expertises are working in this lab for development of feed formulation software and feed portal. The recent research work carried out in this laboratory is listed below:
This laboratory was established to cater the requirements of feed quality and safety under feed resources group. The laboratory has facilities for undertaking basic nutritional analysis of feeds and biological materials. This laboratory has equipments like Gas Chromatograph fitted with electron capture detector and flame ionization detector; Compound microscope with image analysis software; Refrigerated Centrifuge; MilliQ Water purification system; Gerhardt Nitrogen analyser; Soxhtherm (Rapid fat analyzer); CEM Microwave Muffle furnace.
Following are the salient accomplishments:
The laboratory was established in 2008 to carry out advanced research in the areas of nutraceuticals particularly prebiotic, probiotic, gut microflora and alternate feed additives. The current facilities of the laboratory includes High Performance Liquid Chromatography, PCR, Laminar Flow, Nano volume spectrophotometer, High Speed Refrigerated Centrifuge, Ultra-sonic bath, Thin layer chromatography, spray drying etc. As a result of dedicated efforts of the team members, the laboratory was successful in obtaining grants from several funding bodies and successfully completed six extramural research projects. The research achievements of the laboratory were adjudged as "ICAR- Outstanding Interdisciplinary Team Research Award" in 2014. The recent research investigations carried out in the laboratory are outlined below:
The laboratory was established in 2010 to cater the advanced requirements of Omics technologies in existing research work and mandated to work on application of high throughput technologies. The laboratory is equipped to handle transcriptomics, metagenomics and proteomics protocols and bioinformatics. The recent research investigations carried out in the laboratory are mentioned below:
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